Table 2.

Interaction of YidC with periplasmic chaperones and leader peptidase

A non-UV–irradiated (not cross-linked, −UV) and a UV-irradiated (cross-linked, +UV) sample of YidC(K249pBpa) purified from whole cells was separated on a 5–15% SDS gel, and the proteins were visualized by Coomassie staining. The −UV and the +UV lanes were cut into equal slices followed by in-gel trypsin digestion and mass spectrometry. Shown are the quantification for PpiD, Skp, FkbA, and LepB.

YidC(K249pBpa) in vivo cross-links
Proteina	Molecular massb	Gel molecular massc	Relative intensity (−UV/+UV)d	Coveragee	Peptidesf
	kDa	kDa		%
PpiD	68.1	168	0.02	48.6	24
Skp	16	79	0.00	37.3	7
FkbA	28.9	116	0.00	3.7	1
LepB	35.9	133	0.00	36.7	13

^a This is the protein identified.

^b This is the calculated molecular mass.

^c The molecular mass of the gel slice was determined by extrapolation.

^d The relative intensity observed in gel slices from the control lane (−UV) compared with the +UV lane is shown.

^e The sequence coverage of the total sequence was by detected peptides.

^f This is the number of peptides detected.