Figure 10.

Effect of salicylamine analogues in scavenging ONE. A, molecular structures of salicylamine and various analogues (F-SAM, Cl-SAM, PPM). B, effect of analogues in preventing ONE-induced HDL protein crosslinking as shown by Coomassie Blue G250–stained SDS-PAGE gel of unmodified and 1 eq ONE-modified HDL. C, demonstration of PPM (10 eq) preventing ONE-induced HDL dysfunction in protecting against LPS-stimulated TNFα expression in thioglycollate-elicited peritoneal macrophages, whereas its inactive analogue, pentylpyridoxine (PPO), did not. Statistical significance was determined by one-way ANOVA with Dunnett's multiple comparisons compared with LPS + 1 eq ONE + HDL. Results from three individual experiments with three wells per treatment are plotted as mean ± S.D. *, p < 0.05; **, p < 0.01.