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. 2019 Oct 30;294(50):19022–19033. doi: 10.1074/jbc.RA119.009424

Figure 3.

Figure 3.

ONE crosslinks HDL proteins beginning at 0.1 molar eq to apoA-I. A–D, crosslinking of HDL proteins by ONE was determined by (A) Coomassie Blue staining and (B–D) immunoblotting (IB) against apoA-I, apoA-II, and apoA-IV. HDL isolated from normal healthy subjects by DGUC was subjected to ex vivo modification of ONE. SDS-PAGE and Western blots are representative of experiments performed three times.