Figure 4.

A and B, ONE does not significantly alter HDL size or distribution as indicated by (A) size-exclusion chromatography and by (B) Native PAGE gel electrophoresis. HDL was subjected to ex vivo modification of ONE under aqueous conditions 37 °C overnight. HDL was subjected to FPLC using the Superdex 200 column using A280 monitoring. Protein concentration of fractions was assessed using the Bradford assay. Values are represented as mean ± S.D. Statistical significance was calculated by 2-way ANOVA with Dunnett's multiple comparisons test compared with HDL. Asterisk represents statistical significance between HDL and IsoLG-HDL; # represents significance between HDL and ONE-HDL. #, p < 0.05; **, p < 0.01; ***, p < 0.005; ****, p < 0.001. Native PAGE gel electrophoresis was run under standard conditions and the gel was stained with Coomassie Blue G250 to visualize particles.