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Modification of HDL by ONE inhibits the exchangeability of apoA-I on HDL. HDL-apoA-I exchange was analyzed by EPR as described in “Experimental procedures.” Reactions were performed at a constant apoA-I concentration of 1 mg/ml. Samples were assayed in triplicate. Results are plotted as mean ± S.D. Statistical significance was calculated by one-way ANOVA with Dunnett's multiple comparisons compared with unmodified HDL (control). *, p < 0.05; ****, p < 0.0001.
