Figure 6.

Modification of HDL by ONE does not affect cholesterol efflux from apoE-deficient murine macrophages. HDL isolated from normal healthy subjects by DGUC was subjected to ex vivo modification of ONE. Macrophage cholesterol efflux was assessed using thioglycollate-induced macrophages harvested from the peritoneum of apoE-deficient mice and loaded with ³H-cholesterol and acetylated LDL. Vehicle denotes cell culture media with no HDL added to the cells. Efflux of ³H to unmodified and modified HDL was calculated based on radioactive counts in the supernatant after 4 h and normalized to the HDL control. Results from three individual experiments with replicate wells per treatment are plotted as mean ± S.D.