Table 1.
Summary of all the identified peptides and ONE adducts resulting from three independent experiments
The identified residues include ketoamide adducts (+154.0994 atomic mass units) on Lys-12, Lys-23, Lys-96, and Lys-226 in human apoA-I in triplicate experiments. One of the three experiments also identified ketoamide adducts on Lys-94 and Lys-118, and another experiment identified Michael adducts (+156.1200 atomic mass units) on Lys-133 and Lys-226. Ka, ketoamide adduct; MA, Michael adduct. *, represents detection in a single experiment; ***, represents consistent detection in triplicate experiments.
| Lysine | Peptide + adduct | Frequency |
|---|---|---|
| Lys-12 | VKDLATVYVDVLK + 1Ka | *** |
| Lys-23 | DLATVYVDVLKDSGR + 1Ka | *** |
| Lys-94 | DLEEVKAK + 1Ka | * |
| Lys-96 | AKVQPYLDDFQK + 1Ka | *** |
| Lys-118 | QKVEPLR + 1Ka | * |
| Lys-133 | AELQEGARQK + 1MA | * |
| Lys-226 | QGLLPVLESFKVSFLSALEEYTK + 1Ka | *** |
| Lys-226 | QGLLPVLESFKVSFLSALEEYTK + 1MA | * |