Figure 3.
Localization of outer segment-resident proteins in Cep290fl/fl;Cre+ mice. Localization of RHO (A), PRPH2 (B), ROM1 (C), ABCA4 (D), PDE6B (E), GUCY2D (F), ATP8A2 (G), and CNGA1 (H) was probed in Cep290+/fl;Cre+ (at P20) and Cep290fl/fl;Cre+ (at P20 and P40) retinas. I, retinal sections of Ift88+/fl;Cre+ (at P22) and Ift88fl/fl;Cre+ (at P22 and P40) mice were immunostained with anti-RHO antibody and shown for comparisons with Cep290fl/fl;Cre+ mice. Red brackets indicate outer segments. Sections were counterstained with DAPI to show nuclei (blue). At least three animals, both male and female, were used per group, and representative images are shown. Scale bar represents 50 μm. J, length of the outer segment in Cep290fl/fl;Cre+ and Ift88fl/fl;Cre+ retinas. Lengths of the outer segment were measured in areas 0.5–1.0 mm away from the optic nerve. Data are from five animals per genotype (two sections per animal). Mean and standard deviation (S.D.; error bar) are shown. Asterisk indicates statistical significance (two-tailed, two-sample t test assuming unequal variances; p < 0.01).