Figure 8.
Mitochondrial membrane potential analysis. ΔΨm was measured in various cell lines using a fluorescence probe JC-10 assay system. The ratios of fluorescence intensity excitation/emission = 490/590 and 490/530 nm (FL590/FL530) were recorded to delineate the ΔΨm level of each sample. The relative ratios of FL590/FL530 geometric mean between mutant and control cell lines were calculated to reflect the level of ΔΨm. Shown are relative ratios of JC-10 fluorescence intensity at excitation/emission = 490/530 nm and 490/590 nm in the absence (A) and presence (B) of 10 μm FCCP. The average of three determinations for each cell line is shown. Graph details and symbols are explained in the legend to Fig. 2.