Figure 9.

Measurement of ROS. Shown is the ratio of geometric mean intensity between the levels of ROS generation in the vital cells with or without H₂O₂ stimulation. The rates of total ROS production in various cell lines were analyzed by measurement of fluorescence using a BD Accuri C6 flow cytometer system. A, flow cytometry histogram showing fluorescence of cell lines without (red) or with (black) H₂O₂ stimulation. B, relative ratios of fluorescence intensity were calculated in the absence and presence of H₂O₂. The average of three determinations for each cell line is shown. Graph details and symbols are explained in the legend to Fig. 2.