Figure 2.

Structural features of Sec14 orthologs. A, α-carbon backbones of the basic folds of open conformers of Sec14 orthologs from the indicated fungal species: S. cerevisiae (blue); C. albicans (orange); Neurospora crassa (pink); C. dubliniensis (magenta); C. glabrata (yellow); K. lactis (cyan); A. nidulans (brown); and S. pombe (teal). At center is a structural overlay of the collective α-carbon backbones onto that of Sec14 that highlights the high overall conservation of the basic fold across large evolutionary distances. B, electrostatic potential surfaces of open conformers Sec14 and Sec14 orthologs from the indicated fungal species are shown. The en face orientation illustrates the open gating helices and the exposed lipid-binding cavities. Surfaces with electropositive potential are rendered blue, and electronegative surfaces are highlighted in red.