Figure 3.

Structural features of Sec14 paralogs, the Sec14-like Sfh proteins. A, α-carbon backbones of the basic folds of open conformers of Sec14 and the Sec14-like Sfh PITPs from S. cerevisiae: Sec14 (blue); Sfh1 (orange); Sfh2 (pink); Sfh3 (magenta); Sfh4 (cyan); and Sfh5 (teal). At center is a structural overlay of the collective α-carbon backbones superimposed onto that of Sec14 that highlights the overall conservation of the basic Sec14-fold across the yeast Sfh1 family. B, electrostatic potential surfaces of open conformers Sec14 and indicated Sfh PITPs are shown. The en face orientation illustrates the open-gating helices and the exposed lipid-binding cavities and is labeled as “front,” and the opposite side of the molecule is identified as “back.” Surfaces with electropositive potential are rendered blue, and the electronegative surfaces are highlighted in red.