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. 2019 Nov 5;294(50):19081–19098. doi: 10.1074/jbc.RA119.011153

Figure 4.

Figure 4.

Structural barcodes for PtdIns- and PtdCho-binding in Sec14 orthologs and Sec14-like Sfh proteins. A, structural overlay of the PtdIns-binding (left panel) and PtdCho-binding barcode residues (right panel) from Sec14 orthologs superimposed onto those of Sec14. PtdIns-binding barcode residues are shown in blue stick model with PtdIns rendered in green ball and stick. The PtdCho-binding barcode residues are shown in orange stick model, and PtdCho is rendered in green ball and stick. Both barcodes are structurally preserved in Sec14 orthologs across large evolutionary distances. B, structural overlay of the PtdIns-binding (left panel) and PtdCho-binding barcode residues (right panel) from the indicated Sec14-like Sfh PITPs superimposed onto those of Sec14. PtdIns- and PtdCho-binding barcode residues are shown in a stick model using the color code of Fig. 3A to identify residues of individual Sfh proteins. PtdIns is rendered in green ball and stick. The PtdCho-binding barcode regions are shown using the color code of Fig. 3A to identify residues of individual Sfh proteins. PtdCho is rendered in green ball and stick. Although the PtdIns-binding barcodes are structurally well-preserved in the Sfh proteins, the PtdCho barcode is highly divergent (with the exception of Sfh1).