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. 2019 Nov 14;294(50):19306–19321. doi: 10.1074/jbc.RA119.011160

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© 2019 Xu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — ACAT1/2 overexpression resulted in accumulation of free cholesterol on the LD surface in adipocytes. A, the localization of free cholesterol in adipocytes on day 8 of differentiation upon ACAT1/2 overexpression. Bars, 10 μm. Inset, bars, 5 μm. B, quantification of free cholesterol-LD colocalization in A. Two-tailed Student's t test was used (minimum to maximum, line at median; n = 15 cells). **, p < 0.01; ***, p < 0.001. C, the localization of free cholesterol in mature adipocytes transiently overexpressing ACAT1/2. Immunofluorescence was carried out with anti-FLAG primary antibody on cells expressing FLAG-tagged ACAT1/2, but not on EV control cells. Bars, 10 μm. Inset, bars, 1 μm. D, quantification of free cholesterol-LD colocalization in C. Two-tailed Student's t test was used (minimum to maximum, line at median; n = 10 cells). **, p < 0.01.