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. 2019 Nov 14;294(50):19306–19321. doi: 10.1074/jbc.RA119.011160

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© 2019 Xu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 7. — Cholesterol depletion enhanced free cholesterol accumulation on LD surface in adipocytes. A and B, cholesterol depletion by HPCD in FBS medium (A) and HPCD in LPDS medium (B) promoted free cholesterol accumulation on the LD surface in mature adipocytes stably overexpressing ACAT1/2. Bars, 10 μm. Inset, bars, 1 μm. C, quantification of free cholesterol-LD colocalization in A and B. One-way analysis of variance was used (minimum to maximum, line at median; n = 10 cells). **, p < 0.01; ****, p < 0.0001.