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. 2019 Nov 14;294(50):19306–19321. doi: 10.1074/jbc.RA119.011160

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© 2019 Xu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 9. — ACAT1/2 overexpression impaired cholesterol homeostasis in adipocytes. A and B, the level of free cholesterol (FC) (A) and the mRNA level of Hmgcr in (B) mature adipocytes stably overexpressing ACAT1/2. ROSI, rosiglitazone. C and D, the free cholesterol level (C) and the mRNA level of Hmgcr (D) in mature adipocytes transiently overexpressing ACAT1/2. E and F, the free cholesterol level (E) and the mRNA level of Hmgcr (F) in preadipocytes stably overexpressing ACAT1 and ACAT2. 200 μm oleate was added to cells for 20 h before harvest. G and H, HMGCR inhibition restored the free cholesterol level in preadipocytes and adipocytes stably overexpressing ACAT1 and ACAT2. Before harvest, the cells were washed once with PBS followed by the treatment of LPDS medium for 20 h. For preadipocytes, 200 μm oleate was added to cells with LPDS medium to induce LDs for 20 h. Two-tailed Student's t test was used (means ± S.D.; n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, no significance.