Figure 2.
Identification of S45F mutant β-catenin41–49-HLA-A*03:01–specific phage clones. A, five phage clones bind specifically to the β-catenin mutant pHLA-A3 by ELISA. pHLA-A3 complexes or streptavidin bound to plates were incubated with dilutions of the various phage clones (1:500, 1:2,500, and 1:12,500 from darkest to lightest in each color series), followed by detection with an anti-M13 antibody. Data are represented as mean ± S.D. (error bars). B, five phage clones bind specifically to mutant pHLA-A3 on the cell surface. T2A3 cells were peptide-pulsed with β2-microglobulin in the presence or absence of the specified peptide and then stained with the various phage clones and analyzed by flow cytometry. No significant binding was detected to any of the seven Blast peptides.