Figure 5.

Mutational effects on thermostability. The plots show the apparent T_m of copper-saturated SmLPMO10A, ScLPMO10C WT, M2, M5, and M18. The derivative of the fluorescence signal (−dRFU/dT, where RFU is relative fluorescence units) is plotted as a function of the temperature (39). The reactions contained 0.1 g/liter LPMO and were heated from 25 to 99 °C, at a rate of 1.5 °C/min, in the presence of a fluorescent dye (SYPRO Orange). The scans were performed four times for each protein, and the figures show a typical scan. All apparent T_m values had standard deviations below ±0.1 °C.