Figure 7.

Effect of single mutations on chitin degradation by M18. ScLPMO10C WT, M18, and the four M18 mutants were incubated at 1 μm with 10 g/liter β-chitin at 40 °C in 50 mm sodium phosphate buffer (pH 6.0) in the presence of 1 mm ascorbic acid for 24 h. SmChiC was also added, at 0.25 μm, to obtain more rapid solubilization of LPMO-generated oxidized chain ends from the insoluble substrate. The reaction was stopped by filtration, and soluble oxidized products were degraded to oxidized dimers with chitobiase prior to analysis. All reactions were performed in triplicates. The error bars show ± S.D. (n = 3).