(A) CLDN4 gene expression levels in laser capture microdissected specimens of human high grade serous ovarian tumors compared to isolated normal human ovarian surface epithelial cells [36]. Western blot analysis of claudin-4 protein levels in human patient samples of normal fallopian tube and ovarian cancer (B) as well as high grade serous ovarian tumor cell lines (C), using GAPDH as a loading control. Mean ± s.e.m., n=10 HOSE, n=10–53 HGSOC tumor specimens, n=7 other EOC subtypes, *p<0.05, ***p<0.0001.