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. 2019 Dec 13;12:10975–10987. doi: 10.2147/OTT.S216997

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© 2019 Han et al.

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miR-449a mimics and miR-449a inhibitor had influenced the gene expression both in SMMC-7721 and HCCLM3 cell lines. (A) The putative sequences of miR-449a and Notch1 with one binding site. (B) miR-449a significantly inhibited the luciferase activity of the wild-type reporter for Notch1; however, miR-449a did not inhibit the luciferase activity of the reporter vector containing the mutant binding sites of Notch1 in SMMC-7721. (C) The relative levels of miR-449a were up-regulated after transfected with miR-449a mimics while was down-regulated after transfected with miR-449a inhibitor compared with their own NC vector in both cell lines. (D) The relative levels of Notch1 mRNA were up-regulated after being transfected with miR-449a inhibitor and were down-regulated in miR-449a mimics group compared with their own NC vector in both cell lines. (E, F) The expression levels of Notch1 protein were up-regulated after being transfected with miR-449a inhibitor while was down-regulated in miR-449a mimics group compared with their own NC vector in SMMC-7721(E) and HCCLM3(F). *p<0.05, **p<0.01, and ***p<0.001 compared with the control group.