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Experimental timeline. In all groups, timed-pregnant dams received injections of either LPS or saline into the right uterine horn at E15. The offspring in experiment 1 were culled at E17 (48 hr following LPS or saline injection) to assess cytokine mRNA expression. The offspring in experiments 2–5 were used to assess mRNA expression of immune factors, anxiety-like behaviors and MPB protein expression. Only one offspring per sex per litter was used for each assessed endpoint.
