Figure 4. Inactivation of Sesn2 diminishes phosphorylation of AKT.

(A) The sections of lung tissue from Sesn2^+/+;Kras^LSL-G12D and Sesn2^-/-;Kras^LSL-G12D mice were analyzed by immunohistochemistry with antibodies against phospho-AKT and phospho-S6 proteins. (B) The quantification of the intensity of the staining by the program ImageJ presented in relative units (r.u.). The data represent the mean ± S.D. P values were calculated using a two-tailed student’s t-test. (C) Western blot analysis of expression of SESN1 and SESN2 and phosphorylation of AKT and S6 in control A549 cells and cells where SESN1 and SESN2 were inactivated by 2 different CRISPR/Cas9 constructs. (D) Quantification of the relative intensity of phospho-AKT (Thr374) and phospho-S6 (Ser235/236). P values were calculated using one-way ANOVA followed by Tukey’s test for multiple comparisons (n = 3); n.s. – not significant.