Table 1.
Model/Condition | Method | Observation | Citation |
---|---|---|---|
Rat I/R | Microfil | 30-50% reduction | (Basile et al 2001)24 |
Rat I/R | CD31/PECAM staining Cablin staining | ~30% reduction by Cablin staining, preserved by VEGF 121 | (Basile et al 2011)26, (Leonard et al 2008)33 |
Rat I/R | CD31 staining | 90% loss of CD31 staining SS31 | (Liu et al 2014)35 |
Rat I/R | RECA staining | Reduced by > 50%, preserved by EPC microvesicles | (Cantaluppi et al 2012)36 |
Rat I/R | JG12 staining | ~50% reduction; exacerbated by reduced renal mass | (Polichnowski et al 2014)16 |
Mouse I/R | Flurescence micro-angiography | 40% reduction in capillaries at 8 weeks; reduced size of remaining vessels. ~60% decrease in total perfusion area | (Kramann et al 2014)25 |
Mouse I/R | Meca-32 staining and Micro-CT | 20-43% reductions; tight correlation with Meca-32 and Micro-CT; inversely correlated with collagen I staining | (Ehling et al 2016)32 |
Mouse I/R | Cablin staining | 30-40% after 4 weeks MMP9 loss prevented loss of cablin staining following IR | (Lee et al 2011)37 |
Mouse I/R | CD31 staining | Reduction in staining by 20%, preserved by MSC | (Xing et al 2014)38 |
Mouse I/R | CD31 staining | 15-20% rarefaction; some evidence that endothelial proliferation is enhanced in Id1 overexpressing mice | (Lee et al 2011)39 |
DTR transgenic mouse | CD31 staining | Dramatic reduction in CD31 staining associated with fibrosis | (Grgic et al 2012)40 |
Human DGF | CD31/CD34 co-staining | Reduced peritubular capillaries in biopsy 3 months post DGF | (Steegh et al 2011)30 |
Human DGF | vWF staining | Evidence of peritubular capillary endothelial cells damage in sustained acute renal failure | (Kwon et al 2008)29 |
Abbreviation: I/R, ischemia reperfusion; PECAM, platelet endothelial cell adhesion molecule; VEGF, vascular endothelial growth factor; RECA, rat endothelial cell antigen; EPC, endothelial progenitor cells; JG12, endothelial aminopeptidase P in renal tissue; Meca-32, endothelial cell antigen; Micro-CT, micro computed tomography; MMP-9, matrix metalloproteinase-9; MSC, mesenchymal stem cells; Id1, inhibitor of DNA-binding/differentiation proteins; DTR, diphtheria toxin receptor; DGF, delayed graft function, Vwf, von Willebrand Factor.