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. Author manuscript; available in PMC: 2020 Oct 31.
Published in final edited form as: Cell. 2019 Oct 31;179(4):953–963.e11. doi: 10.1016/j.cell.2019.10.009

Figure 4. Parental H3 nucleosome segregation is replication dependent.

Figure 4.

(A) Experimental timeline whereby ESCs are synchronized at G1/S phase (0 hr) and released to continue the cell cycle or treated with 1 μg/ml aphidicolin to block S-phase progression. Cells were harvested at 12 hr and 24 hr. (B-E) Native biotin ChIP-qPCR analysis of the Hoxc6 peak primer set 22 (B), Pou5f1 peak primer set 9 (C), Pou5f1 peak primer set 22 (D), and Pou5f1 peak primer set 45 (E) in cells treated (green) or not treated (black) with aphidicolin following biotin enrichment during G1 synchronization and release into S-phase. All biotin enrichment levels are relative to input and normalized to Drosophila chromatin spike-in followed by subtraction of the minus-Dox (-Dox) control. Biotin-enriched aphidicolin chromatin was then normalized to biotin-H3 at 0 hr. Error bars represent standard error of three biological replicates. Statistical significance was determined using 2way ANOVA (***p<0.001 and **** p<0.0001).