| Antibodies |
| Rabbit monoclonal anti c-Myc (MYC) |
Cell Signaling |
Cat# 5605; RRID:AB_1903938 |
| Mouse monoclonal anti α-Tubulin |
Millipore Sigma |
Cat# T6199; RRID:AB_477583 |
| Rabbit monoclonal anti eIF5A |
Abcam |
Cat# ab32407; RRID:AB_2230904 |
| Rabbit polyclonal anti DOHH |
Millipore Sigma |
Cat# HPA041953; RRIDABJ0795386 |
| Rabbit polyclonal anti Hypusine |
Millipore Sigma |
Cat# ABS1064; RRID:AB_2631138 |
| Rabbit polyclonal anti phospho-eIF2α |
Cell Signaling |
Cat# 9721; RRID:AB_330951 |
| Rabbit polyclonal anti eIF2α |
Cell Signaling |
Cat# 9722; RRID:AB_2230924 |
| Chemicals, Peptides, and Recombinant Proteins |
| FuGENE® HD Transfection Reagent |
Promega Corporation |
Cat# E2311 |
| Hygromycin B |
Thermofisher Scientific |
Cat# 10687010 |
| Puromycin (Puromycin Dihydrochloride) |
Thermofisher Scientific |
Cat# A11138–03 |
| Polybrene Infection/Transfection Reagent |
Millipore Sigma |
Cat# TR-1003-G |
| TRIzol |
Thermofisher Scientific |
Cat# 15596026 |
| Critical Commercial Assays |
| In-Fusion® HD Cloning Kit |
Takara (Clontech) |
Cat# 639649 |
| DNeasy Blood & Tissue Kit |
QIAGEN |
Cat# 69504 |
| Agencourt AMPure XP |
Beckman Coulter Life Sciences |
Cat# A63882 |
| Qubit dsDNA BR Assay Kit |
Thermofisher Scientific |
Cat# Q32853
|
| Bioanalyzer High Sensitivity DNA Analysis |
Agilent |
Cat# 5067–4626 |
| KAPA Library Quantification Kit for Illumina platforms (KK4835) |
Kapa Biosystems (Roche) |
Cat# 07960204001 |
| Direct-zol RNA Miniprep Kit |
Zymo Research |
Cat# R2051 |
| SuperScript III First-Strand Synthesis SuperMix |
SuperScript III First-Strand Synthesis SuperMix |
Cat# 18080400 |
| SYBR Green PCR Master Mix |
Thermofisher Scientific |
Cat# 4309155 |
| Lipofectamine RNAiMAX Transfection Reagent |
Thermofisher Scientific |
Cat# 13778075 |
| Phusion® High-Fidelity DNA Polymerase |
New England Biolabs |
Cat# M0530S |
| QuikChange Lightning Site-Directed Mutagenesis Kit |
Agilent |
Cat# 210518 |
| NEBuilder HiFi DNA Assembly Master Mix |
New England Biolabs |
Cat# E2621S |
| Dual-Luciferase Reporter Assay System |
Promega Corporation |
Cat# E1910 |
| Deposited Data |
| CRISPR screening data |
This paper |
GEO: GSE132010
|
| Raw and processed ribosome profiling data |
This paper |
GEO: GSE132010
|
| RNA-seq data (complementary to ribosome profiling) |
This paper |
GEO: GSE132010
|
| Experimental Models: Cell Lines |
| HCT116 |
ATCC |
CCL-247 |
| HEK293T |
ATCC |
CRL-3216 |
| HCT116 MYC 5’ UTR reporter |
This paper |
N/A |
| HCT116 EGFP reporter |
This paper |
N/A |
| Oligonucleotides |
| Sequences of oligonucleotides used in this study provided in Table S3
|
This paper |
N/A |
| Recombinant DNA |
| AAVS1 hPGK-PuroR-pA donor |
Addgene (Rudolf Jaenisch) |
Plasmid #22072 (Hockemeyer et al., 2009) |
| pMSCV-Hygro |
Clontech |
Cat# 634401 |
| pEGFP-N1 |
Clontech |
Cat# 6085–1 |
| pAAVS-EGFP-DONOR |
This paper |
N/A |
| pAAVS-5’ UTR-DONOR |
This paper |
N/A |
| Human CRISPR Knockout Pooled Library (GeCKO v2) |
Addgene (Feng Zhang) |
Pooled Library #1000000048, #1000000049 (Sanjana et al., 2014) |
| lentiCRISPR v2 |
Addgene (Feng Zhang) |
Plasmid #52961 (Sanjana et al., 2014) |
| psPAX2 |
Addgene (Didier Trono) |
Plasmid #12260 |
| pMD2.G |
Addgene (Didier Trono) |
Plasmid #12259 |
| pGL3-Control |
Promega Corporation |
Cat# E1741 |
| pGL3-Control_NP2 |
This paper |
N/A |
| pRL-SV40 |
Promega Corporation |
Cat# E2231 |
| pcDNA3.1(+) |
Invitrogen |
Cat# V790–20 |
| Software and Algorithms |
| Bowtie2 |
Langmead and Salzberg, 2012 |
N/A |
| MAGeCK |
Li etal., 2014 |
N/A |
| HISAT2 |
Kim et al., 2015 |
N/A |
