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. Author manuscript; available in PMC: 2020 Feb 26.
Published in final edited form as: Methods Enzymol. 2019 Feb 26;618:1–27. doi: 10.1016/bs.mie.2019.01.003

Figure 2:

Figure 2:

Chromatogram (230 nm) from HPLC purification of H2B-UbDCA by semipreparative C4 RP-HPLC. Crosslinked complexes comprised solely of ubiquitin elute in a single sharp peak, with H2B-UbDCA eluting in a broad peak late in the gradient. Fractions can be lyophilized and run on a gel to confirm separation.