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. Author manuscript; available in PMC: 2020 Feb 26.
Published in final edited form as: Methods Enzymol. 2019 Feb 26;618:1–27. doi: 10.1016/bs.mie.2019.01.003

Figure 4:

Figure 4:

Representative HPLC chromatogram (230 nm) from purification of H2BUb by semipreparative C4 RP-HPLC. Ligated product (H2BUb) elutes as the major product, Ub thioester (Ub-MPAA) and hydrolyzed Ub (Ub-OH) elute earlier in the gradient. (#) - MPAA thiol used in ligation reaction.