Table 2. Protein secretion pipeline applied to the D. vitifoliae salivary gland proteome.

Secretory prediction pipeline	Protein number	Percentage
Identified proteins	1511	100,00%
TMHMMs	240	15,88%
GPI-anchors	23	1,52%
Proteins without structural retaining signals	1259	82,32%
Classical secretion pathway	151	10,19%
Non-classical secretion pathway	354	23,43%
Secreted proteins	420	27,80%

The secretion pipeline used to identify secreted proteins within the salivary gland proteome of D. vitifoliae larvae feeding on root galls of Teleki 5C (V. berlandieri x V. riparia). The online tools TMHMM Server 2.0 (TMHMMs), PredGPI (GPI-anchors), SignalP Server 5.0 (classical secretion pathway) and SecretomeP Server 2.0 (non-classical secretion pathway) were used in a stepwise fashion to determine the likelihood of proteins to be secreted.