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. 2019 Sep 3;21(12):1540–1551. doi: 10.1093/neuonc/noz116

Fig. 2.

Fig. 2

Histologic characterization of orthotopic PDX tumors. (A–G) Representative images of MAF-811. (A) Axial section of mouse brainstem showing P2 MAF-811 xenograft tumor in fourth ventricle (black square, injection site) and temporal horn of lateral ventricle (green square, metastatic lesion). (B–C) MAF-811_XC xenograft showing classic features of ependymoma: small round blue cells with stippled chromatin pattern, forming true ependymal rosettes (arrows) and pseudorosettes (arrowheads). (D) Immunohistochemical staining against GFAP showing diffuse positivity in tumor cells. (E) MAF-811 xenograft metastases to lateral ventricle. (F) Original patient tumor from MAF-811_5 showing xenograft had high degree of morphologic similarity with original. (G–K) Representative images of MAF-928_XC. G. Whole mount of mouse cerebellum showing ependymoma (square). (H) Leptomeningeal spread of the tumor (arrows). (I) Higher magnification view of xenograft showed homogeneous tumor with tumor cells arranged around blood vessels (perivascular pseudorosettes). (J–K) Tumor cells were positive for GFAP and exhibited dot-like (arrows) positivity on EMA immunohistochemical staining. (L) Original patient tumor from MAF-928_2. Scale bars indicated on images.