Figure 2. C-DIM12 mediated activation of Nr4a rescues long-term memory deficits in aged mice.

(A) Strong memory procedure to test memory rescue by C-DIM12 (n=10) compared to vehicle (n=11) in aged mice, with C-DIM12 drug delivery 4 days preceding training and on the training day. (B) Total distance travelled in the open-field during habituation (t₍₁₉₎=0.8397, p=0.4115). (C) Total time spent in inner and outer zones of the open-field during habituation session (Two-way ANOVA: Treatment × zones interaction F (1, 19) = 0.1406, p=0.7119. (D) Preference for displaced object (DO) in a strong-training SOR task in aged mice receiving either vehicle or C-DIM12 drug is shown as % discrimination for the displaced object compared to the non-displaced object. Long-term memory was assessed 24-hours after initial training, and aged mice in the C-DIM12 treatment group showed greater discrimination for the displaced object, whereas the vehicle treated mice displayed nearly no % discrimination for the displaced object (Two-way ANOVA: Treatment × Sessions interaction F _{(1, 19)} = 6.716, p= 0.0179, Sidak’s post-hoc tests: **p = 0.01 comparing C-DIM12 Vs vehicle during the 24hr test, **p < 0.01 comparing C-DIM12 training Vs C-DIM12 24hr test). (E) Total exploration was similar during the test for both the groups (t ₍₁₉₎= 0.3681, p=0.7168).