Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Staphylococcus aureus) | SaCas9 | Addgene | pX601, Cat. #: #61591 |
|
| Peptide, recombinant protein | SpCas9 | NEB | Cat. #: M0386 | |
| Cell line (Homo-sapiens) | HFF (Hs27) | ATCC | Cat# CRL-1634, RRID:CVCL_0335) |
|
| Cell line (Homo-sapiens) | U2OS | ATCC | Cat# HTB-96, RRID:CVCL_0042 |
|
| Cell line (Chlorocebus sabaeus) | Vero | ATCC | Cat# CCL-81, RRID:CVCL_0059 |
|
| Antibody | Anti-ICP8 (Rabbit serum) |
Knipe et al., 1987 | 1:5000 | |
| Antibody | Anti-ICP4 (Mouse monoclonal, purified from hybridoma cell line 58S (ATCC HB8183)) |
Showalter et al., 1981 | 1:2000 | |
| Antibody | Anti-ICP27 (Mouse monoclonal) |
Eastcoast Bio | Cat. #: P1119 | 1:5000 |
| Antibody | Anti-GAPDH ([6C5], Mouse monoclonal) |
Abcam | Cat. #: ab8245 | 1:10000 |
| Recombinant DNA reagent | Addgene | lentiCRISPRv2, Cat #: 52961 | Cloning vector | |
| Recombinant DNA reagent | pX601-AAV-CMV-SaCas9-T2A-mCherry | This paper | Template of SaCas9-T2A-mCherry for lentiSaCas9-mCherry-Puro |
|
| Recombinant DNA reagent |
lentiSaCas9-mCherry-Puro | This paper | Cloned SaCas9 gene into lentiCRISPRv2 | |
| Software, algorithm | ICE analysis toolbox |
https://ice.synthego.com | ||
| Software, algorithm | bcbio-nextgen | https://github.com/bcbio/bcbio-nextgen | v1.15 | |
| Software, algorithm | MuTect2 | https://www.ncbi.nlm.nih.gov/pubmed?term=20644199 | v2 | |
| Software, algorithm |
bwa-mem | https://arxiv.org/abs/1303.3997 | v0.7.17 | |
| Software, algorithm | Cas-OFFinder | https://www.ncbi.nlm.nih.gov/pubmed/24463181 | v2.4 |