Table 1. CRISPR/Cas9 target sequences.
| Name | Efficiency of cleavage | SaCas9 sgRNA + PAM (g was added as needed) |
Target sequences (GenBank: KT899744) |
|---|---|---|---|
| UL30 | |||
| UL30-1 | + | GCGTCCCGACTGGGGCGAGGT AGGGGT | 62811–62831 |
| UL30-2 | ++ | gAAGTTTTGCCTCAAACAAGGC GGGGGT | 62779–62799 |
| UL30-3 | - | GCGGCGTGGACCACGCCCCGG CGGGGT | 63060–63080 |
| UL30-4 | ++ | gTGCCCCCCCGGAGAAGCGCG CCGGGGT | 62923–62942 |
| UL30-5 | ++ | gACACGTGAAAGACGGTGACG GTGGGGT | 63097–63116 |
| UL30-6 | + | gACCAGCCGAAGGTGACGAAC CCGGGGT | 63595–63614 |
| UL30-7 | ++ | GGCCATCAAGAAGTACGAGGG TGGGGT | 63532–63552 |
| UL30-24* | ++ | gAAACCCCAAAAGCCGCTTGGG TGGGAT | 62589–62609 |
| UL30-25* | + | gCCACCCGAACCCCTAAAGAGG GGGGAT | 62637–62657 |
| UL30-26* | - | gCATGCCGGCCCGGGCGAGCCT GGGGGT | 62542–62562 |
| UL30-27* | ++ | gCCATCCCACCCAAGCGGCTTT TGGGGT | 62581–62601 |
| UL29 | |||
| UL29-1 | ++ | gTCAAGGTCCCCCCCGGGCCCC TGGGAT | 61861–61881 |
| UL29-2 | + | GTGTTTGAGGTCGCCGGGCCG GGGGGT | 61502–61522 |
| UL29-3 | ++ | GCCAGCCAGGGTAAGACCCCG CGGGGT | 61028–61048 |
| UL29-4 | ++ | GCCGCCGTCGCGCCCACCCCG CGGGGT | 61007–61027 |
| UL29-14* | ++ | GAGGGTGGGAGACCGGGGTTG GGGAAT | 62029–62049 |
| UL29-15* | ++ | GTCGGGCGTCCGTCGTCGTGC CGGGAT | 61952–61972 |
| UL29-16* | ++ | gCGGGGGTTGTCTGTGAAGGGT AGGGAT | 62064–62084 |
| UL29-17* | ++ | gATCGGCACCCCGTGGTTACCC GGGGGT | 62084–62104 |
| UL29-18* | ++ | gCAGACAACCCCCGGGTAACCA CGGGGT | 62072–62092 |
| UL29-19* | ++ | GGACCCCGCGTTGCCAGCCGC CGGGGT | 62113–62133 |
| UL29-20* | ++ | GAACCCCGGCGGCTGGCAACG CGGGGT | 62105–62125 |
| UL29- 21 | ++ | GGTTCTCGCACGACGGGGCTC GGGGGT | 61685–61705 |
| UL54 | |||
| UL54-1* | ++ | GCTGTCGGCTGCCGTCGGGGC TGGGGT | 113541–113561 |
| UL54-2 | ++ | gACCTGGAATCGGACAGCAAC GGGGAGT | 113667–113687 |
| UL54-3 | ++ | GCTCCGGTCCGTCCTCTCCGT GGGGGT | 113728–113748 |
| UL54-4 | ++ | GCGTCTGGGTGCTGGGTACGC CGGGGT | 113803–113823 |
| UL54-5 | ++ | GGCGGACGCCGTGGGCGTCGC AGGGGT | 113982–114002 |
| UL54-6 | ++ | gTGGTTCTGGGGGCACGCCGGC GGGGGT | 114055–114075 |
| UL54-7 | ++ | GCAGGCTGGGCTTTGGTCGGT GGGGGT | 113957–113977 |
| UL54-8 | ++ | gCGCCGTGGGCGTCGCAGGGGT CGGGGT | 113988–114008 |
| UL54-9 | + | GTCCGTCCACCCCGCCCCGGGG CGGGGT | 114098–114119 |
| UL54-14* | ++ | gCGCTTCCGCGGGGACCCGGGC GGGGGT | 113234–113254 |
| UL54-15* | ++ | gCGCCCGGGGGGCGGAACTAGG AGGGGT | 113347–113367 |
| UL54-16 | ++ | GGCGGCTCTCCGCCGGCTCGG GGGGGT | 113641–113661 |
| Rs1 | |||
| Rs1-1 | ++ | GCCGGGCGTCGTCGAGGTCGT GGGGGT | 130775–130795, 146992–147012 |
| Rs1-2 | - | gCCGCTCGTCGCGGTCTGGGCT CGGGGT | 130866–130886, 146901–146921 |
| Rs1-3 | - | GGGGGTGGTCGGGGTCGTGGT CGGGGT | 130796–130816, 146971–146991 |
| Rs1-4 | ++ | gATCGTCGTCGGCTAGAAAGGC GGGGGT | 130599–130619, 147168–147188 |
| Rs1-5 | ++ | GGCGCGGCGACAGGCGGTCCG TGGGGT | 130475–130495, 147292–147312 |
| Rs1-6 | ++ | GCGAGGCCGCGGGGTCGGGCGT CGGGAT | 130634–130655, 147132–147153 |
| Rs1-7 | + | GGGTCCGGGGCGGCGAGGCCG CGGGGT | 130622–130642, 147145–147165 |
| Rs1-8 | ++ | gCGCGAGGCGCGGGCCGTCGGG CGGGGT | 130290–130310, 147477–147497 |
| Rs1-9 | ++ | GCGGACGACGAGGACGAGGACC CGGAGT | 130378–130399, 147388–147409 |
| Rs1-15* | ++ | GCCGATGCGGGGCGATCCTCC GGGGAT | 130954–130974, 146813–146833 |
| Rs1-16* | - | gTACGCGGACGAAGCGCGGGAG GGGGAT | 131142–131162, 146625–146645 |
| Rs1-17* | + | gCGCGTCGACGGCGGGGGTCGT CGGGGT | 131061–131081, 146706–146726 |
| Rs1-18* | ++ | GCGCTAGTTCCGCGTCGACGGC GGGGGT | 131070–131091, 146696–146717 |
| UL26-27 | ++ | GAGGAAATCGGCACTGACCAA GGGGGT | 52742–52762 |
| UL37-38 | ++ | GTATAACACCCCGCGAAGACG CGGGGT | 84066–84086 |
++:full cleavage (no residual substrate DNA on agarose gel), +: partial cleavage (some residual substrate DNA on agarose gel), -: no cleavage, *: non-coding region.