Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent | Mus Musculus (Mouse strain) | (Nowotschin et al., 2013) | CBF1:H2B-Venus | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Kisanuki et al., 2001) | Tie2-Cre | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Wu et al., 2012) | Nfatc1-Cre | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Wang et al., 2010) | Pdgfb-iCreERT2 | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Wang et al., 2010) | Cdh5-CreERT2 | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Koch et al., 2008) | Dll4flox | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Mancini et al., 2005) | Jag1flox | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Grunwald et al., 2004) | Efnb2flox | |
| Genetic reagent | Mus Musculus (Mouse strain) | (Radtke et al., 1999) | Notch1flox | |
| Genetic reagent | Mus Musculus (Mouse strain) | (D’Amato et al., 2016) | MfngGOF | |
| Sequenced-based reagent | FH1_DLL4 | 5’-GTTACACAGTGAAAAGCCAG-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Sequenced-based reagent | RH1_DLL4 | 5’-CTCTCCTCTGATATCAAACAC-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Sequenced-based reagent | FH1_JAG1 | 5’-ACTACTACTATGGCTTTGGC-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Sequenced-based reagent | RH1_JAG1 | 5’-ATAGCTCTGTTACATTCGGG-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Squenced-based reagent | FH1_HEY1 | 5’-CCGGATCAATAACAGTTTGTC -3’ | KiCqStart_ SIGMA qPCR primer |
|
| Sequenced-based reagent | RH1_HEY1 | 5’-CTTTTTCTAGCTTAGCAGATCC-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Sequenced-based reagent | FH1_EFNB2 | 5’-AAAGTTGGACAAGATGCAAG-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Sequenced-based reagent | RH1_EFNB2 | 5’-TGTACCAGCTTCTAGTTCTG-3’ | KiCqStart_ SIGMA qPCR primer |
|
| Transfected construct (human) |
VSV-G | Viral Vectors Unit, CNIC, Spain | Lentiviral construct to transfect shRNA |
|
| Cell line (include species here) | HUVEC | Lonza | ||
| Transfected construct (mouse) | pRLL-IRESeGFP | Addgene |
|
Lentiviral construct to transfect eGFP or Full-length murine EphrinB2 |
| Transfected construct (human) | shRNA to JAG1 | SIGMA | SHCLNG-NM_000214 Clone ID:NM_000214.2-3357s21c1;Clone ID:NM_000214.2-1686s21c1 | transfected construct (human) |
| Transfected construct (human) | shRNA to DLL4 | SIGMA | SHCLNV-NM_019074 Clone ID:NM_019074.2-2149s21c1; Clone:NM_019074.2-2276s21c1 |
transfected construct (human) |
| Antibody | Anti-CD31/Pecam1 (Monoclonal Rat) |
BD Biosciences Pharmingen |
550274 MEC13.3 | IF=1:100 |
| Antibody | Dll4 (Polyclonal Rabbit) |
Santa Cruz Biotechnology | Sc-28915 | IF=1:100 |
| Antibody | Endomucin (Polyclonal Rat) |
Santa Cruz Biotechnology |
sc-65495 V.7C7 | IF=1:200 |
| Antibody | Jag1 (Monoclonal Rabbit) |
Cell Signaling Technology |
2620 28H8 |
IF=1:100 |
| Antibody | NFATc1 (Monoclonal Mouse) |
Enzo Life Sciences |
ALX- 804-022-R100 7A6 |
IF=1:100 |
| Antibody | Cleaved Notch1 (Val1744) (Monoclonal Rabbit) |
Cell Signaling Technology | 4147 D3B8 | IF=1:100 |
| Antibody | p27 (Polyclonal Mouse) |
Medical and Biological Laboratories |
K0082-3 p27 Kip1 | IF=1:100 |
| Antibody | Notch 3 (Polyclonal Rabbit) |
Abcam | ab23426 G00041 | IF=1:100 |
| Antibody | ERG (Monoclonal Rabbit) |
Abcam | ab110639 EPR3863 |
IF=1:100 |
| Antibody | Glut1 (Polyclonal Rabbit) |
MERCK Millipore |
07-1401 C00222 | IF=1:100 |
| Antibody | BrdU (Monoclonal Mouse) |
BD Biosciences | 347580 B-44 |
IF=1:50 |
| Antibody | Isolectin IB4- Alexa Fluor 647 |
Molecular Probes |
I32450 | IF=1:300 |
| Antibody | Biotin Anti-rat |
Vector laboratories |
BA4001 | IF=1:200 |
| Antibody | Biotin Anti-rabbit |
Jackson | 111-066-003 | IF=1:200 |
| Antibody | Anti-mouse Alexa Fluor 647 |
Jackson | 115-606-003 | IF=1:200 |
| Antibody | Anti-mouse Alexa Fluor 488 |
Invitrogen | A-11029 | IF=1:200 |
| Antibody | Anti-rat | Invitrogen | A-11006 | IF=1:200 |
| Peptide, recombinant protein | Human-Vegf165 | Human Peprotech |
100-20 | 10ng/ml |
| Commercial assay or kit | Click-iT EdU Imaging Kit | Thermo Fisher Scientific | C10340 | |
| Commercial assay or kit | HydroxyprobeTM-1 Plus Kit |
Hydroxyprobe, Inc (HPI). | (Pimonidazole Hydrochloride CAS#70132-50-3) | |
| Other | Matrigel | Corning | 354234 | |
| Software | Cutadapt v1.6 | (Martin, 2011) | ||
| Software | RSEM v1.2.20 | (Li and Dewey, 2011) | ||
| Software | Limma | (Ritchie et al., 2015) | Bioconductor package | |
| Software | GOplot | (Walter et al., 2015) | ||
| Software | IPA | http://www.ingenuity.com |