Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifier | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | Opti-MEM | Thermo fisher Scientific |
Cat#11058021 | |
| Chemical compound, drug | Lipofectamine 2000 TransfectionReagent | Thermo fisher Scientific |
Cat#11668027 | |
| Chemical compound, drug | Dulbecco’s Modified Eagle’s Medium - high glucose | MERCK | Cat#D6429 | |
| Chemical compound, drug | Fetal Bovine Serum (FBS) | MERCK | Cat#F7524 | |
| Chemical compound, drug | MEM Non-essentialAmino Acid Solution(100x) | MERCK | Cat#M7145 | |
| Chemical compound, drug | Poly-D-lysine hydrobromide | MERCK | Cat#P6407 | |
| Chemical compound, drug | Penicillin-Streptomycin | MERCK | Cat#P4333 | |
| Chemical compound, drug | HEPES solution | MERCK | Cat#H0887 | |
| Chemical compound, drug | Sodium pyruvatesolution | MERCK | Cat# S8636 | |
| Chemical compound, drug | MicroScint-20 | PerkinElmer | Cat# 6013621 | |
| Chemical compound, drug | Iron-55 Radionuclideas Ferricchloride/s.A.>3 Ci/g,Ferric chloride in 0.5MHCl | ANAWA Biomedical Servicesand Products | Cat# ARX-0109–2 | |
| Chemical compound, drug | Terrific Broth (TB) medium | Sigma | Cat#T9179 | |
| Chemical compound, drug | Ampicillin | AppliChem | Cat#A0839 | |
| Chemical compound, drug | L-(+)-Arabinose | Sigma-Aldrich | Cat#A3256 | |
| Chemical compound, drug | Lysozyme | AppliChem | Cat#A3711 | |
| Chemical compound, drug | DNase I | AppliChem | Cat#A3778 | |
| Chemical compound, drug | n-decyl-β-D-maltoside (DM) | Anatrace | Cat#D322 | |
| Chemical compound, drug | n-dodecyl-β-D-maltoside (DDM) | Anatrace | Cat#D310 | |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) | Avanti Polar Lipids, Inc | Cat#850757 | |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) | Avanti Polar Lipids, Inc | Cat#840457 | |
| Chemical compound, drug | Diethyl ether | Millipore Sigma | Cat#296082 | |
| Chemical compound, drug | Triton X-100 | Millipore Sigma | Cat#T9284 | |
| Chemical compound, drug | Calcein | Thermo Fisher Scientific | Cat#C481 |
|
| Chemical compound, drug | Valinomycin | Thermo Fisher Scientific | Cat#V1644 | |
| Chemical compound, drug | A-23187 Free Acid (Calcimycin) | Thermo Fisher Scientific | Cat#A1493 | |
| Peptide, recombinant protein | HRV 3C protease | Expressed andpurified (Expression vector pET-3C) RaimundDutzler laboratory | N/A | |
| Commercial assay or kit | Corning96 Well Black PolystyreneMicroplate | MERCK | Cat# CLS3603 | |
| Commercial assay or kit | PfuUltra High-fidelityDNA Polymerase | Agilent Technologies | Cat# 600380 | |
| Commercial assay or kit | Nickel NTA Agarose Resin | ABT Agarose Bead Technologies | 6BCL-NTANi-X | |
| Commercial assay or kit | Superdex 200 10/300 GL | GE Healthcare | Cat#17517501 | |
| Commercial assay or kit | 24-well Cryschem M crystallization plate | Hampton Research | Cat#HR1-002 | |
| Commercial assay or kit | Bio-Beads SM-2 | Bio-Rad | Cat#1523920 | |
| Commercial assay or kit | Avestin LiposoFastLiposome FactoryBasic | Millipore Sigma | Cat#Z373400 | |
| Commercial assay or kit | 400 nm polycarbonatefilters for LiposoFast | Millipore Sigma | Cat#Z373435 | |
| Commercial assay or kit | 96-well black-walled microplate | Thermo Fisher Scientific | Cat#M33089 | |
| Commercial assay or kit | Amicon 50 kDa MWCO centrifugal filter | EMD Millipore | Cat#UFC805024 | |
| Commercial assay or kit | 0.22 µm Ultrafree-MC Centrifugal Filter | EMD Millipore | Cat#UFC30GV | |
| Strain, strain background | Escherichia coli MC1061 | Thermo Fisher Scientific | Cat#C66303 | |
| Strain, strain background | Eremococcus coleocola strain | https://www.dsmz.de/collection | DSM No. 15696 | |
| Cell line (human) | HEK-293 | ATCC | CRL-1573 | |
| Cell line (human) | HEK-293T | ATCC | CRL-3216 | |
| Recombinant DNA | Human SLC11A2 isoform 1A-IRE (+) ORF inserted in pBluescript SK - vector | Hentze laboratory- EMBL, Heildelberg, Germnay | N/A | |
| Recombinant DNA | pIRES2 DsRed-Express2 Vector | Takara Clontech | Cat# 632540 | |
| Recombinant DNA | Expression vector pBXC3GH | Addgene | Cat#47070 | |
| Recombinant DNA | Expression vector pBXC3H | Addgene | Cat#47068 | |
| Recombinant DNA | Expression vector pET-3C | Dr. Arie Geerlof, EMBL Munich | N/A | |
| Software, algorithm | XDS | Kabsch, 2010 | http://xds.mpimf-heidelberg.mpg.de/ | |
| Software, algorithm | CCP4 | Collaborative Computational Project, 1994 | http://www.ccp4.ac.uk/ | |
| Software, algorithm | Coot 0.8.9 | Emsley and Cowtan, 2004 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |
| Software, algorithm | PHENIX 1.17 | Adams et al., 2002 | http://phenix-online.org/ | |
| Software, algorithm | MSMS | Sanner et al., 1996 | http://mgltools.scripps.edu/packages/MSMS/ | |
| Software, algorithm | DINO 0.9.4 | http://www.dino3d.org | http://www.dino3d.org | |
| Software, algorithm | LIGPLOT | Wallace et al., 1995 | https://www.ebi.ac.uk/thornton-srv/software/LIGPLOT/ | |
| Software, algorithm | CHARMM | Brooks et al., 1983 | https://www.charmm.org/charmm/ | |
| Software, algorithm | SWISS-MODEL | Biasini et al., 2014 | https://swissmodel.expasy.org/ | |
| Software, algorithm | Prism 8.3.0 | GraphPad | https://www.graphpad.com/ | |
| Software, algorithm | MicrocalOrigin 5.0 | Microcal Software, Inc. | http://www.microcal.com | |
| Software, algorithm | MicroCal Concat ITC | Malvern Panalytical | https://www.malvernpanalytical.com/en/ |