Table 1. Transport kinetics and inhibition.

Protein	Inhibitor	KM (µM)	vmax*	Ki (µM)	α
		Michaelis-Menten		Equation 2
hDMT1	-	3.1 ± 0.6	4.6 ± 0.3	0.57 ± 0.17	>100
	1 µM Br-DBFIT	9.2 ± 1.2	4.2 ± 0.2
	2.5 µM Br-DBFIT	14.7 ± 2.8	4.3 ± 0.4
	5 µM Br-DBFIT	14.4 ± 3.0	3.4 ± 0.4
hDMT1	-	2.6 ± 0.5	2.6 ± 0.1	0.35 ± 0.07	17.4
	0.25 µM TMBIT	4.0 ± 0.4	2.3 ± 0.1
	0.5 µM TMBIT	6.1 ± 0.8	2.4 ± 0.1
	1.25 µM TMBIT	8.0 ± 1.0	2.1 ± 0.1
hDMT1	-	2.9 ± 0.4	3.1 ± 0.1	0.08 ± 0.01	>100
	0.25 µM TEBIT	13.1 ± 3.3	3.0 ± 0.4
	0.5 µM TEBIT	18.3 ± 5.3	2.9 ± 0.5
	1.25 µM TEBIT	26.8 ± 8.6	2.5 ± 0.5
hDMT1	-	2.6 ± 0.5	2.6 ± 0.1	3.6 ± 0.7	13.5
	5 µM Br-BIT	6.7 ± 0.7	2.4 ± 0.1
	10 µM Br-BIT	7.4 ± 1.2	2.2 ± 0.1
	25 µM Br-BIT	12.4 ± 3.7	1.6 ± 0.2
hDMT1 S476V	-	2.3 ± 0.5	-	-	-
hDMT1 N520L	-	2.4 ± 0.5	-	-	-
hDMT1 F523A	-	2.7 ± 0.6	-	-	-
EcoDMT	-	4.3 ± 0.5	21.9 ± 0.5	14.2 ± 2.6	>100
	10 µM Br-BIT	13.7 ± 1.2	21.7 ± 0.4
	50 µM Br-BIT	21.7 ± 3.6	21.4 ± 1.0
	100 µM Br-BIT	31.5 ± 6.9	20.4 ± 1.3
EcoDMT N456A	-	12.5 ± 1.7	19.1 ± 0.6	29.3 ± 7.1	8.5
	10 µM Br-BIT	17.8 ± 4.0	18.3 ± 1.1
	50 µM Br-BIT	28.8 ± 5.4	14.9 ± 0.8
	100 µM Br-BIT	33.9 ± 6.9	13.8 ± 0.8
EcoDMT N456L	-	7.5 ± 0.7	17.3 ± 0.4	28.8 ± 5.3	29.2
	10 µM Br-BIT	13.1 ± 1.7	16.2 ± 0.5
	50 µM Br-BIT	19.0 ± 2.4	13.8 ± 0.5
	100 µM Br-BIT	27.0 ± 3.9	15.1 ± 0.6
EcoDMT N456A S459A Q463A		7.2 ± 0.9	21.9 ± 0.5	23.8 ± 3.2	>100
	10 µM Br-BIT	15.7 ± 1.7	21.7 ± 0.4
	50 µM Br-BIT	32.3 ± 4.5	21.4 ± 1.0
	100 µM Br-BIT	47.9 ± 5.6	20.4 ± 1.3

^*ν_max values for hDMT1 measurements are given in pmol min⁻¹ well⁻¹ and νmax values for EcoDMT measurements in ΔF Δt⁻¹.