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. Author manuscript; available in PMC: 2019 Dec 17.
Published in final edited form as: Biochemistry. 2017 Jun 29;56(27):3475–3483. doi: 10.1021/acs.biochem.7b00339

Figure 3.

Figure 3

Functional validation and cross-linking of hCNT3 cysteine mutants. A) Schematic of the experimental design. Mutations are shown as X and O. Sites of potential cross-linking are indicated with a red highlight and a thick black line. B) Uridine uptake inhibition studies in PK15NTD cells transiently transfected with hCNT3 constructs. C) Western blot of the cysteine cross-linking experiment involving V274C/A285C mutants, D) Y266C/F463C mutants, E) T297C/A456C mutants, and F) A440C/A456C mutants.