Skip to main content
. Author manuscript; available in PMC: 2019 Dec 17.
Published in final edited form as: Sci Signal. 2018 Apr 10;11(525):eaal3606. doi: 10.1126/scisignal.aal3606

Fig. 7. Trans-presentation of IL-15 by IL-15Rα augments the activation of NK cells by ULBP2, but not MICA.

Fig. 7

(A) Representative TIRF and dSTORM images of membrane-proximal F-actin in pNK cells incubated for 10 min at 37°C on slides coated with PLL, unloaded IL-15Rα, IL-15–loaded IL-15Rα, or MICA or ULBP2 in the presence of ICAM-1, with or without IL15Rα-IL-15. Scale bars, 4 μm. Regions outlined by the white squares (middle row) are magnified (bottom row). Scale bars, 500 nm. An example of an actin mesh “hole” is indicated in red. (B) Histogram of the percentage of cells with dense peripheral actin rings. pNK cells were incubated as described in (A). F-actin was visualized using fluorescently labelled phalloidin, and the percentages of cells forming dense peripheral F-actin rings were quantified from confocal images. Data are from 100 to 500 cells from at least two independent donors. Bars represent means ± SD. (C) Flow cytometric analysis of CD107a surface abundance on pNK cells incubated for 5 hours at 37°C on slides coated as described in (A). Data are from one experiment and are representative of five experiments. (D) Percentage of pNK cells that degranulated as calculated from the flow cytometric data. Bars represent means ± SD from five donors. Each color represents one donor. *P < 0.05 and ****P < 0.0001 by one-way ANOVA with Tukey`s post-hoc test. ns, not significant.