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Transcriptional regulators drive lifestyle changes in Gram-positive pathogens. (A) In C. difficile, the intracellular levels of Spo0A~P regulate the lifestyle switch to form spores or biofilms and (B) two different forms of the transcriptional regulator PrfA in L. monocytogenes, are required to activate the intracellular lifestyle or to form in vivo aggregates.
