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. 2019 Dec 11;11:343. doi: 10.3389/fnagi.2019.00343

FIGURE 1.

FIGURE 1

Histological analysis for neurofibrillary pathology in the hippocampus of SHR72 and W72 rats after inoculation of insoluble tau. (A) Immuno-blot using pan-tau antibody DC25 showing insoluble tau isolated from human AD brain (AD-PHF). Recombinant human 2N4R (Tau 40) was used as positive control. Histological images showing AT8-positive structures in the hippocampus of W72 (B, H–L) and SHR72 transgenic line (C, M–Q). (E, F) The presence of neurofibrillary pathology was confirmed using a pT212 phospho-tau specific antibody in the hippocampus of the transgenic lines. A statistical analysis did not reveal any difference in the levels of AT8-positive (D) or pT212 positive (G) structures in the hippocampus of the two transgenic lines (unpaired t-test: AT8 p = 0.818; pT212 p = 0.999). However, AT8-positive structures were prominent in the CA3 region of the W72 line (J) when compared to the SHR72 line (O). There was not difference in the levels of AT8-positive structures in CA1 (H,M), CA2 (I,N), or dentate gyrus (K,P). The brainstem of the transgenic lines was used as a positive control for the presence of genuine AT8-positive structures (L,Q). Scale bar B,C,E,F: 500 μm and H–Q: 50 μm.