Figure 6.

Effect of heat stress on NLR pyrin domain containing 3 (NLRP3), caspase-1, and interleukin-1β (IL-1β) protein expression. (A) Cells were incubated at different temperatures (37, 39, 41, and 43°C) for 45 min. NLRP3, caspase-1, and IL-1β were measured by western blot. (B) Cells were subjected to heat stress (43°C) for different times (0, 15, 30, and 45 min). NLRP3, caspase-1, and IL-1β are measured by western blot. Levels of NLRP3, caspase-1, and IL-1β protein expression are shown in the bar graph. Values are mean ± SD for six independent experiments. ^*p < 0.05, ^**p < 0.01 compared with the control group (37°C) or sham heated group.