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. 2019 Dec 11;10:2862. doi: 10.3389/fimmu.2019.02862

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Copyright © 2019 Wang, Shen, Li, Luo, Tan, He, Zhang, Yang, Liu, Wang, Tang, Luo and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondrial reactive oxygen species (ROS) mediate heat stress-induced pyroptosis in splenic lymphocytes in vitro. (A) Cells were subjected to heat stress (43°C) for 45 min with or without ROS scavengers [Mito-TEMPO or N-acetyl cysteine (NAC)]. In the other groups, cells were incubated at 37°C and treated with Mito-TEMPO or NAC or YVAD. Representative flow cytometry results of TEMPO and NAC on heat stress-induced double positivity for caspase-1 (vertical) and SYTOX Green (horizontal). (B) Quantification of the membrane pore formation (SYTOX Green staining, horizontal) and caspase-1 activation cells was calculated from (A). Values are mean ± SD for six independent experiments. ^**p < 0.01 compared with the control group at 37°C. ##p < 0.01, compared with the control group at 43°C.