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. 2019 Dec 11;10:2810. doi: 10.3389/fimmu.2019.02810

Figure 2.

Figure 2

Verification of the interaction between gD and viperin. (A–C) Co-IP of viperin and gD from the lysates of transfected cells. HEK293T cells were co-transfected with plasmids combination pViperin-Flag/pcDNA3.1-gD (A), Flag vector/pcDNA3.1-gD (B), or pViperin-Flag/pcDNA3.1 (C). Twenty-four hours post-transfection, cells were lysed and immunoprecipitated with mouse anti-Flag mAb or mouse IgG control. Immunoprecipitated proteins, as well as the cell lysates, were separated in denaturing 10% SDS-PAGE, and analyzed by IB with mouse anti-Flag mAb or mouse anti-gD mAb. (D) Co-IP of viperin and gD from the lysates of HSV-1 infected cells. HEK293T cells transfected with pViperin-Flag for 24 h were infected with HSV-1 BAC Luc at an MOI of 1 for 16 h. Then, cells were lysed and Co-IP assays were carried out, and analyzed by IB with mouse anti-Flag mAb or anti-gD mAb.