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. 2019 Dec 11;10:2810. doi: 10.3389/fimmu.2019.02810

Figure 6.

Figure 6

gD suppresses IRAK1 and TRAF6 interaction in the presence of viperin. (A,B) HEK293T cells were co-transfected with the expression plasmids of IRAK1-HA and TRAF6-myc, along with pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag. Twenty-four hours post-transfection, cells were collected, and followed by Co-IP with mouse anti-Myc mAb. IB analysis was developed with the indicated antibodies. Densitometry of the IRAK1 and TRAF6 interaction bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments, and statistical analyses were performed using one-way ANOVA. **P < 0.01.