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. 2019 Dec 11;7:402. doi: 10.3389/fbioe.2019.00402

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Copyright © 2019 Shridhar, Amsden, Gillies and Flynn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The DTB has a distinct ECM structure and composition as compared to DAT. (A) Picrosirius red staining and the resulting birefringence visualized under a polarized light microscope indicated predominantly thicker fibrillar collagen types in the DTB, and thinner fibers in the DAT. (B) Higher levels of collagen and sulphated GAG content were measured in the DTB as compared to the DAT (*p < 0.05, n = 3, N = 3 pooled). (C) Immunohistochemical staining and semi-quantitative comparative analyses of the DTB and DAT displayed significantly higher amounts of collagen IV, VI, and laminin in the DAT. Fibronectin was qualitatively more abundant in the DAT, with regional variability leading to no significant difference between the groups (n = 3, N = 2 tissue donors, 4–5 images per sample) (*p < 0.05) Scale bar: 100 μm.