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. 2019 Dec 11;7:402. doi: 10.3389/fbioe.2019.00402

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Copyright © 2019 Shridhar, Amsden, Gillies and Flynn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation that the MCS, MCS+DAT and MCS+DTB hydrogel systems exhibited similar bulk properties. (A) Confocal image analysis showing that the ECM particles (blue) were well distributed throughout the hydrogel phase. Scale bars: 1 mm. No significant differences were observed between the three hydrogel systems in terms of (B) gel content (n = 3 samples per group/trial, N = 3 trials) and (C) Young's moduli, measured under unconfined compression in PBS at 37°C (N = 5).