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. 2019 Dec 11;7:402. doi: 10.3389/fbioe.2019.00402

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Copyright © 2019 Shridhar, Amsden, Gillies and Flynn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High cell viability and similar cell densities within the hydrogels were confirmed under all media conditions tested. (A) Cell viability of ASCs encapsulated within MCS, MCS+DAT, MCS+DTB hydrogels under proliferation, adipogenic, and osteogenic media conditions maintained >90% cell viability over the 14 d culture period. (B) Analysis of total live cell number indicated no significant differences between any of the groups under all media conditions, eliminating cell density as a factor mediating differentiation (n = 3 samples per group/trial, N = 3 trials with different ASC donors).