Table 3.
Antiviral activity of ribavirin against FMDV O (in terms of log10TCID50/mL with 95% confidence interval) at different timed samples using (a) cell bio-assay, (b) sandwich ELISA, (c) PCR and (d) SYBR Green based real time PCR
| Assays | Time of harvest (h) | Ribavirin concentration (μg/50 μL) | |||
|---|---|---|---|---|---|
| 5 | 10 | 15 | Virus control | ||
| log10TCID50/mL | |||||
| Cell bioassay | 6 | 0 | 0 | 0 | 3.30 (2.07–4.53) |
| 12 | 3.80 (3.09–4.51) | 3.80 (3.09–4.51) | 2.80 (2.09–3.51) | 5.30 (4.07–6.53) | |
| 24 | 6.05 (5.11–6.99) | 4.80 (3.79–5.81) | 3.80 (3.09–4.51) | 6.80 (5.93–7.67) | |
| 48 | 5.80 (5.09–6.51) | 4.80 (4.09–5.51) | 4.05 (3.11–4.99) | 7.80 (6.07–8.53) | |
| Sandwich ELISA | 6 | 4.80 (4.09–5.51) | 4.80 (4.09–5.51) | 4.55 (3.61–5.49) | 4.80 (3.93–5.67) |
| 24 | 7.55 (6.61–8.49) | 6.55 (5.61–7.49) | 4.80 (4.09–5.51) | 7.30 (6.07–8.53) | |
| 48 | 6.80 (6.09–7.51) | 5.80 (5.09–6.51) | 5.80 (5.09–6.51) | 7.30 (6.07–8.53) | |
| PCR | 6 | 5.80 (5.09–6.51) | 5.63 (4.81–6.45) | 5.55 (4.61–6.49) | 5.80 (4.93–6.67) |
| 12 | 7.80 (7.09–8.51) | 6.30 (5.14–7.46) | 5.80 (5.09–6.51) | 7.30 (6.07–8.53) | |
| 24 | 7.30 (6.18–8.42) | 7.55 (6.61–8.49) | 6.80 (5.79–7.81) | 7.30 (6.07–8.53) | |
| 48 | 7.30 (6.14–8.46) | 7.55 (6.61–8.49) | 7.55 (6.61–8.49) | 7.30 (6.07–8.53) | |
| Real time PCR | 24 | 7.80 (7.09–8.51) | 7.05 (6.11–7.99) | 6.80 (5.79–7.81) | 7.30 (6.07–8.53) |
| 48 | 7.80 (6.79–8.81) | 7.55 (6.61–8.49) | 6.80 (6.09–7.51) | 7.30 (6.07–8.53) | |