Table 1.
Oligonucleotides used for qRT-PCR
| Genes | GenBank accession # | Oligonucleotide 5′–3′ |
|---|---|---|
| Alkaline phosphatase, liver/bone/kidney, ALPL | NM_000478.4 |
CATGCTGAGTGACACAGACAAGAA ACCGCCCACCACCTTGTAG |
| Bone gamma-carboxyglutamate protein, BGLAP | NM_199173.4 |
GGTGCAGAGTCCAGCAAAGG GCGCCTGGGTCTCTTCACTA |
| Bone morphogenetic protein 1, BMP1 | NM_006129.4 |
AGGGCAATGATGTGTGCAAGT GCCATGCAGCTTGGAGTCA |
| bone morphogenetic protein receptor, type IA, BMPR1A | NM_004329.2 |
TCAGCGAACTATTGCCAAACAG TTGCCCATCCATACTTCTCCAT |
| Collagen, type I, alpha 1, COL1A1 | NM_000088.3 |
CGATGGCTGCACGAGTCA CAGGCGGGAGGTCTTGGT |
| Collagen type III alpha 1, COL3A1 | NM_000090.3 |
TGGAGGATGGTTGCACGAA GTAGTCTCACAGCCTTGCGTGTT |
| Epidermal growth factor receptor, EGFR | NM_005228.3 |
CTGAGCTCTCTGAGTGCAACCA CAGCTTTGCAGCCCATTTCT |
| Fibroblast growth factor 2, FGF-2 | NM_002006.4 |
CGGTCAAGGAAATACACCAGTTG GCCCAGGTCCTGTTTTGGAT |
| Fibroblast growth factor receptor 1, FGFR1 | NM_015850.3 |
TGCACCAACGAGCTGTACATG CTGCTTGAAGGTGGGTCTCTGT |
| Insulin-like growth factor 1, IGF1 | NM_000618.3 |
TGCTTCCGGAGCTGTGATCT GCTGACTTGGCAGGCTTGAG |
| Insulin-like growth factor 1 receptor, IGFR1 | NM_000875.3 |
ACGGCATGGCATACCTCAAC TTCGGCTACCATGCAATTCC |
| Runt-related transcription factor 2, RUNX2 | NM_004348.3 |
GTATGTCCGCCACCACTCACTAC GAAGGGTCCACTCTGGCTTTG |
| Homolog 2, similar to mothers against decapentaplegic, SMAD2 | NM_005901.5 |
GACACCAGTTTTGCCTCCAGTATT TCCAGAGGCGGAAGTTCTGTT |
| Homolog 4, similar to mothers against decapentaplegic, SMAD4 | NM_005359.5 |
CATTGGATGGGAGGCTTCAG TCCAGAGACGGGCATAGATCA |
| Homolog 5, similar to mothers against decapentaplegic, SMAD5 | NM_005903.6 |
AGGAACCTGAGCCACAATGAAC GTTGTTGGGCTGGTGGAAAG |
| Secreted phosphoprotein 1, SPP1 | NM_000582.2 |
TTCGCAGACCTGACATCCAGTA CCATTCAACTCCTCGCTTTCC |
| Transforming growth factor beta receptor 1, TGFBR1 | NM_004612.2 |
TGTGCTTCGTCTGCATCTCACT TGGCACTCGATGGTGAATGA |
| Tumor necrosis factor, TNF | NM_000594.3 |
AGGCCAAGCCCTGGTATGA GCTGAGTCGGTCACCCTTCTC |
| Vitamin D receptor, VDR | NM_000376.2 |
GCTGGACGCCCACCATAA CTCCACCATCATTCACACGAACT |
| Cyclin-dependent kinase inhibitor 1B, CDKN1B | NM_004064.4 |
GCCAGCGCAAGTGGAATTT CTCCACCTCTTGCCACTCGTA |
| TNF receptor associated factor 2, TRAF2 | NM_021138.3 |
GGCCGTCTGTCCCAGTGAT TTCGTGGCAGCTCTCGTATTC |
| Cluster differentiation 40 molecule, CD40 | NM_001250.4 |
ACACTGCCACCAGCACAAATACT CTGTTTCTGAGGTGCCCTTCTG |
| Tumor necrosis factor receptor superfamily, member 1A, TNFRSF1 | NM_003844.3 |
CTGGCGCTTGGGTCTCCTA TGCGTTGCTCAGAATCTCGTT |
| Aldehyde dehydrogenase 1 family member A1, ALDH1A1 | NM_000689.4 |
GCAGTGAAGGCCGCAAGA CCCTCTCGGAAGCATCCATAG |