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. 2019 Dec 17;10:5747. doi: 10.1038/s41467-019-13744-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Cryo-transmission electron microscopy (TEM) images of a virus, b lipid cancer membrane vesicles, and c ExtraCRAd. B16.OVA cell line was employed as source for the membrane. Scale bar: 500 nm. The insets on the right were magnified ten times compared to the original image. b Nano-tracking analysis (NTA) showing the size distribution of the samples. The size distribution is expressed as percentage of the total population. The size intervals are presented on the right. c NTA quantification of the concentration of viral particles or nanosized vesicles in the samples. The results are presented as number × 10⁶ vesicles per ml. The data were analyzed by two-way ANOVA followed by Tukey’s post-test. The level of statistical significance was set at probability level ****p < 0.0001. d Dynamic light scattering (DLS) size analysis of the system in different extrusion buffers. The results are presented as mean ± s.d. (n = 3). e Venn-diagram representing the results retrieved from the mass spectrometry analysis of the samples (virus, ExtraCRAd produced with membranes derived from human lung cancer A549 cells and extruded membrane vesicles from A549 cells). The Venn size reflects the total number of unique proteoforms for each sample, with the shared proteins presented in the intersection of the diagrams. f Graphic illustration of an ExtraCRAd: adenovirus 5-D24-CpG (light blue), lipid membrane (orange), and proteins (light green). The error bars indicate s.d.